RCDA: A highly sensitive and specific alternatively spliced transcript assembly tool featuring upstream consecutive exon structures
نویسندگان
چکیده
منابع مشابه
Alternatively Spliced Human Genes by Exon Skipping - a database
UNLABELLED Alternative splicing of mRNA allows many gene products with different functions to be produced from a single coding sequence. Exon skipping is the most commonly known alternative splicing mechanism. A comprehensive database of alternative splicing by exon skipping is made available for the human genome data. 1,229 human genes are identified to exhibit alternative splicing by exon ski...
متن کاملA Unique, Consistent Identifier for Alternatively Spliced Transcript Variants
BACKGROUND As research into alternative splicing reveals the fundamental importance of this phenomenon in the genome expression of higher organisms, there is an increasing need for a standardized, consistent and unique identifier for alternatively spliced isoforms. Such an identifier would be useful to eliminate ambiguities in references to gene isoforms, and would allow for the reliable compar...
متن کاملEstablishment of a highly sensitive and specific exon-trapping system.
We have established a highly sensitive and specific exon-trapping system (SETS) with a specific plasmid vector in which an exon in a given DNA segment is identified by its ability to remain as a mature mRNA after splicing. The SETS provides us with the isolation of possible exons rapidly and easily from DNA fragments in chromosomal regions of more than 300 kilobase pairs. Genomic DNA fragments ...
متن کاملThe exon trapping assay partly discriminates against alternatively spliced exons.
A cosmid containing eight exons of the gene coding for the microtubule-associated tau protein was subjected to the exon trapping assay. All the constitutive exons contained in the cosmid (4, 5, 7 and 9) were efficiently captured regardless of size. Of the four alternatively spliced exons, three (3, 4A and 8) were not isolated by the assay, but the behavior of exon 6 depended on the identity of ...
متن کاملAn alternatively spliced exon links intermediate filaments to adhesions.
Anchorage of the contractile actomyosin apparatus to the plasma membrane at discrete sites in muscle and non-muscle cells enables the transmission and conversion of force into work, such as muscle contraction and membrane deformation to regulate cell and tissue shape. Assembly, stabilization and turnover of adhesion sites are complex processes that involve structural components, a variety of si...
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ژورنال
عنوان ژورنال: Genomics
سال: 2012
ISSN: 0888-7543
DOI: 10.1016/j.ygeno.2012.08.004